The Institute achieved a major breakthrough in the captive broodstock development, induced breeding and seed production of the Asian seabass Lates calcarifer for the first time in the country. The technology for seabass seed production has been standardized.

Seabass seed produced in the Institute's hatchery were successfully cultured in a trials ponds.

F3 generation broodstock of seabass has been raised for breeding trials.

Captive broodstock of grey mullet Mugil cephalus was developed and maintained. Induced maturation and breeding of M. cephalus under captivity was achieved and the hatchlings were reared upto 8 days.

A viable captive broodstock of grouper Epinephelus tauvina has been built up. Conversion of females into males was successfully done by the administration of 17 Methyl Testosterone. Successful induced breeding of the captive broodstock was done in two trials with the production of hatchlings. Larval rearing is being standardized.

Captive broodstock of penaeid shrimp L. monodon and Fenneropenaeus indicus were maintained under hatchery conditions. Induction of maturity in females and breeding under controlled conditions were achieved.

Marsupenaeus japonicus was successfully reared in captive conditions and F6 generation larvae were obtained.

Demonstration of improved traditional culture of P. monodon in Pokkali fields was carried out under the technical guidance of the Institute. Production of 1100 kg/ha/3 months was achieved.

Improved traditional culture of tiger shrimp Penaeus monodon in tide-fed ponds at the Kakdwip Research Centre of CIBA, yielded production of 2.3 ton of shrimp / ha / 3-4 months.

Successful demonstration of field testing of Vibrio whole cell based shrimp immunostimulant on Penaeus monodon was conducted in farmers fields. P. monodon post larvae fed with heat killed Vibrio sp. resulted in significant increase in growth and survival.

CIBA developed the technology for captive broodstock development, induced maturation, breeding and production of berried females of the mud crabs Scylla tranquebarica and S. serrata .

For the first time the life cycle of S. tranquebarica was completed in captivity. Rearing from zoea to the megalopa stage and subsequently rearing upto post-larval instar stage (baby crab) has been achieved.

Life cycle of S. serrata has also been completed with the production of baby crab.

A technology package was developed for shrimp feed processing and production on commercial scale, using a nutritionally balanced feed formulation suitable for the culture of F. indicus and P. monodon .

Formulated feeds using indigenous feed ingredients were developed for rearing seabass fry, with protein content ranging from 25-40%. The fry were successfully weaned to these feeds. The growth performance was comparable to that obtained from imported feed.

Several plant protein sources such as oil cakes, Spirulinand mushrooms were evaluated for use in shrimp feeds. Feed attractants, growth enhancers and probiotics were tested for use in shrimp feeds and valuable data obtained. Larval and grow-out diets for rearing seabass were prepared and tested in fields.

Extensive investigations were conducted on the white spot virus disease of shrimp. A simple DNA – based diagnostic technique using Polymerase Chain Reaction (PCR) was developed for diagnosis of white spot disease in shrimp. A set of PCR primers were developed (298 base pair) for standardization of molecular diagnosis of the disease. An agreement The technology has been commerialized and the kit in available in the market.

Studies on laboratory induction of white spot disease in decapod crustaceans showed that mud crab Scylla serrata and S. tranquebarica and 3 species of lobsters were found to act as reservoir / asymptomatic carrier hosts for white spot virus.

A simple technique was developed for the cryopreservation of milt of grey mullets Mugil cephalus and Liza macrolepis .

A survey of shrimp farming practices adopted by various categories of farmers was conducted in several maritime states and the socio-economic conditions and extension needs of farmers were assessed.

An extensive computerized database information system was developed on brackishwater fishery resources of the country.

Extensive investigations were conducted on the impact of shrimp farming on the environment. Several extensive, semi-intensive and intensive shrimp farms in Tamil Nadu (Tuticorin) and Andhra Pradesh (Nellore & East Godavari districts) were surveyed and the socio-economic and environmental impacts of shrimp farming were assessed.

Transfer of technology : The benefits of research conducted by CIBA in different areas of shrimp / fish aquaculture have percolated to the farmers / entrepreneurs by means of publication of bulletins and extenstion literature, farmers meets, exhibitions and demonstration programmes. Training programmes on several aspects of brackishwater fish / shrimp / crab seed production and culture are being conducted regularly.

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