Scanning Electron Microscope

Instrument Details:

Model: JSM IT300

Make: JEOL

Working Principle:

The Scanning Electron Microscope (SEM) utilizes electrons to show an enlarged image of a specimen, in the same way as the transmission electron microscope (TEM). Since an electron has a shorter wavelength than light, it enables us to observe smaller things than can be seen with the Optical microscope. The word resolution refers to the smallest size that can clearly be observed (the resolution of the human eye is said to be 0.2mm). The SEM’s resolution is 0.5 to 4nm. SEM provides an enlarged image projected onto a fluorescent screen, by using the electrons which were reflected or generated from the surface of the specimen whereas in TEM the image is formed by the electrons transmitted (passed) through the thinly prepared specimen.

Applications:

The JSM IT300 integrated with secondary electron detector that makes it ideal for studying the samples in biology, taxonomy, anatomy and pathology.  The resolution of SEM is 0.5 to 4nm.  CIBA has expertise on scanning electron microscopy of biological samples and encourages SEM studies related to biological samples.

User Instruction:

  • The samples should be dry and should withstand high vacuum (~ 10 -5 Pa). Users must prepare their samples using appropriate protocols to ensure that the native structure of the organism is retained. Users should care to avoid the introduction of drying artifacts, shrinkage and collapse that can be seen in microphotographs of poorly preserved / processed specimens.
  • Users must take effort to do some research prior to collecting samples and make SEM work easier. Fixation is the first and most important step for optimum preservation of biological samples. Make sure to use the best fixative for collecting and preserving your samples. The sample size for dry samples should be less than diameter: 100 mm Height: 40 mm.
  • Wet biological sample withstand low temperature (-10 C – 20 C) can be analysed.
  • The user is welcome to be present in the laboratory during analysis.

Contact E-mail ID: sathishkumart@ciba.res.in

Contact: 91-044-24610565

Scanning electron Microscopy (SEM) charges excluding the GST

Particulars Description Industry University/ National Lab/R&D
Image analysis Per Sample 7000 3000

Additional Information

  1. How many days before the user Scientist is to be contacted?
  • At least 3 weeks
  1. How the user will be informed?
  • The users will be informed about their date & time of slot by e-mail.
  1. How many days are required to analyse the sample?
  • Image analysis takes one day.